Immunoblot Assay in Determination of Serum Antibody Profile of Helicobacter Pylori Infection

Authors

  • A. Fazeli Medical Sciences of Isfahan University
  • M. Saffari Medical Sciences of Kashan University
  • MA. Motavalii Medical Sciences of Kashan University
Abstract:

A high prevalence of antibody to cag A has been observed in H. pylori seropositive patients (HP+) living in developed countries. This is felt to be correlated with the development of serious sequelae of this infection.  We examined the sero-prevalence of antibody to cag A and other specific H. pylori antigens in symptomatic and asymptomatic individuals living in Kashan.Sera from 37 patients whose HP status was determined by culture, histology, RUT, and ELISA were enrolled in this study.  The Helico – blot 2.1 western blot system was used for the detection of antibodies to specific antigens of H.pylori in sera obtained from both positive and negative HP.Out of 85 patients, 47 (55.3%) were HP+ and 38 (44.7%) HP- .  Among the HP+ group, 37 were tested with immunoblot system.  Patients and their respective clinical disease were composed of duedenitis 19, non-ulcer dyspepsia 4 , dyspepsia 4 , gastritis 24 , cancer and metaplasia 11 and 2 normal biopsies. In that order the corresponding antibodies to cag A was 73%,75%,75%,83%,81.8% and 50%.The results of this study suggest that cag A antibody is more frequent in the acute status; however, this factor is diagnostically insufficient in some disease processes.  In addition,cag A+ phenotype can not be used as a single marker for high risk patients.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

immunoblot assay in determination of serum antibody profile of helicobacter pylori infection

a high prevalence of antibody to cag a has been observed in h. pylori seropositive patients (hp + ) living in developed countries. this is felt to be correlated with the development of serious sequelae of this infection.  we examined the sero-prevalence of antibody to cag a and other specific h. pylori antigens in symptomatic and asymptomatic individuals living in kashan. sera from 37 patients ...

full text

Association between helicobacter pylori infection and serum iron profile

Background: It is clear that Helicobacter pylori (H. pylori) is a spiral bacterium that infects human and causes gastritis and peptic ulcer. The aim of this study was to determine the relationship between H .pylori infection and the iron status of the body. Methods: This study was conducted in Shahid Beheshti Hospital of Babol University of Medical Sciences, Babol, Iran from August 2007 to July...

full text

Serum Level of Helicobacter pylori Antibody in Stroke Patients

Background and Objective: Stroke is the third most common cause of death in developed countries and the most common disabling neurological disease. There are several risk factors for stroke. Regarding this, the present study was conducted to investigate the effect of Helicobacter pylori antibody on the incidence of stroke. Materials and Methods: This case-control study was conducted on 50 pati...

full text

Endoscopic gastritis, serum pepsinogen assay, and Helicobacter pylori infection

Endoscopic findings of the background gastric mucosa are important in the Helicobacter pylori-seroprevalent population. It is strongly correlated not only with the risk of gastric cancer, but also with the excretion ability of gastric mucosa cells. In noninfected subjects, common endoscopic findings are regular arrangement of collecting venules, chronic superficial gastritis, and erosive gastri...

full text

Determination of Helicobacter pylori Antigen in Stool Samples and Comparison with Rapid Urease Test in Patients Suspected of Helicobacter Infection

Background and Aims: Helicobacter pylori (H. Pylori) is the microorganism that infects nearly half of word's population. There are several invasive and non-invasive methods for diagnosis of infection. The main objective of this study was to evaluate antigen of H. Pylori in feces with H. pylori stool antigen (HpSA) test and comparison with rapid urease test (RUT) in the patients suspected to be ...

full text

Comparing Serum Levels of Autophagy-Related Gene 5 in People With and Without Helicobacter Pylori Infection

Background: Laboratory studies have shown that gastric epithelial infection cells with Helicobacter pylori (H. pylori) can increase autophagy. Disruption in this process can cause various diseases, including cancer. Objective: This study aims to compare the serum level of Autophag -Related Gene 5 (ATG5) in individuals with and without H. pylori infection. Methods: This case-control pilot stud...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 28  issue 2

pages  90- 92

publication date 2015-10-28

By following a journal you will be notified via email when a new issue of this journal is published.

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023